Xinhui Nie , Jianli Tu , Bin Wang , Xiaofeng Zhou , Zhongxu Lin .2015.Plos One
Abstract
To provide a resource for cotton genetics and breeding,an interspecific hybridization between Gossypium hirsutumcv. Emian22 and G. barbadense acc. 3-79
was made. A population of 54 BILs
(backcross inbred lines, BC1F8) was developed with the aim of transferring G. barbadense genes into G. hirsutum in order to
genetically analyze these genes' function in a G. hirsutum background and create new germplasms for breeding. Preliminary
investigation of the morphological traits showed that the BILs had diverse
variations in plant architecture, seed size, and fuzz color; the related traits
of yield and fiber quality evaluated in 4 environments also showed abundant
phenotypic variation. In order to explore the molecular diversity of the BIL population, 446 SSR markers
selected at an average genetic distance of 10 cM from our interspecific linkage map were used to genotype
the BIL population. A total of
393 polymorphic loci accounting for 84.4% MAF (major allele frequency) > 0.05 and
922 allele loci were detected, and the Shannon diversity index (I) was 0.417
per locus. The average introgression segment length was 16.24 cM, and an
average of 29.53 segments were introgressed in each BIL line with
an average background recovery of 79.8%. QTL mapping revealed 58 QTL associated
with fiber quality and yield traits, and 47 favored alleles derived from the donor
parent were discovered. This study demonstrated that the interspecific BIL population was
enriched with much phenotypic and molecular variation which could be a resource for cotton genetics and breeding.