Abstract
A population of 178 recombinant inbred lines (RILs)
was developed using a single seed descendant from a cross between G. hirsutum.
acc DH962 and G. hirsutum. cv Jimian5, was used to construct a genetic map and
to map QTL for fiber and yield traits. A total of
644 polymorphic loci were used to construct a final genetic map, containing 616
loci and spanning 2016.44 cM, with an average of 3.27 cM between adjacent
markers. Statistical analysis revealed that segregation distortion in the
intraspecific population was more serious than that in the interspecific population.
The RIL population and the two parents were phenotyped under 8 environments (two
locations, six years), revealing a total of 134 QTL, including 64 forfiber qualities
and 70 for yield components, independently detected in seven environments,
explaining 4.40-15.28% of phenotypic variation (PV). Among the 134 QTL, 9
common QTL were detected in more than one environment, and 22 QTL and
19 new QTL were detected in combined analysis (E9). A total of 26 QTL hotspot
regions were observed on 13 chromosomes and 2 larger linkage groups, and some QTL clusters
related to fiberqualities or yield components were also
observed. The results obtained in the present study suggested that to map
accurate QTL in crops with larger plant types, such as cotton,
phenotyping under multiple environments is necessary to
effectively apply the obtained results in molecular marker-assisted selection
breeding and QTL cloning.
http://www.ncbi.nlm.nih.gov/pubmed/26110526