金双侠-GCGI(Group of Cotton Genetic Improvement)

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基本信息

姓名:	金双侠	出生年月:	1979.2
性别:	男	硕/博导：	博导
民族:	汉	开设课程：	植物生物技术,作物生产实习,育种学实习
职称:	教授	研究方向：	棉花生物技术；细胞质基因组及生物技术
学位:	博士	研究方向：	棉花生物技术；细胞质基因组及生物技术

联系方式

办公电话：027-87283955
电子邮件：jinsx1979@gmail.com

个人简介

华中农业大学植物科学技术学院作物遗传育种专业博士，教授，博士生导师。

1997－2001，华中农业大学农学系农学专业就读；

2001－2006，华中农业大学植物科学技术学院攻读硕士、博士学位，获农学博士学位；

2007.10 －2012.4 在美国 University of Central Florida， Henry Daniell 教授指导下进行博士后研究，研究方向为植物叶绿体转化。

研究领域主要涉及棉花生物技术及细胞质基因组和生物技术研究等。目前主要从事棉花与主要害虫（烟粉虱，盲蝽象，棉铃虫等）互作的分子机制研究以及高效表达载体（叶绿体转化表达体系）的开发及转基因研究。

近年来先后主持国家自然科学基金面上项目3项，转基因专项子课题1项，湖北省自然科学基金重点项目（省杰青）1项，华中农业大学杰青培植项目1项，华中农业大学人才培育专题项目2项。以第一作者或通讯作者在Trends in Plant Science, New phytologist, Plant Physiology, Plant Biotechnology Journal，Scientific Reports, IJBS 等杂志发表论文20篇，总引用次数达到900多次。

科研项目

十三五国家重点研发专项子课题主持 2016-2020

转基因专项子课题主持 2016-2020

湖北省自然科学基金重点项目（杰青项目）主持， 2015.1-2016.12

国家自然科学基金烟粉虱与棉花互作的分子机理研究主持，2014-2017

国家自然科学基金植物凝集素基因在棉花中的功能分析, 主持, 2012-2015

新疆建设兵团博士资金项目转基因抗病虫、抗逆聚合杂交棉新组合选育技术研究，主持，2007－2009

国家自然科学基金棉花外源基因叶绿体转化体系的建立及其应用（30771368），主持，2008－2010

教育部博士点新教师基金以gfp基因为报告基因监测转基因棉花花粉漂移（20070504087），主持，2008－2010

国家自然科学基金重大国际合作项目 Development of strong pest-resistance cotton via transforming plastid and its application in breeding, 主要参加者，2009－2011

国家自然科学基金利用基因诱捕构建棉花功能基因组研究突变群体，主要参加，2005-2007

发明专利及获奖情况

获奖情况：

【棉花种质创新及强优势杂交新品种选育与应用】国家科技进步二等奖 2013年

【棉花体细胞离体遗传操作技术与控制理论】教育部自然科学二等奖 2013年

指导的本科生获【华中农业大学十佳大学生国创项目】 2013年

湖北省优秀博士学位论文（第10批）获奖者，2008年

湖北省优秀本科论文指导奖，2008年。
“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖

获第十、十一届湖北省自然科学优秀论文一等奖2次

申报6项发明专利：

1. 超声波辅助农杆菌转化棉花胚芽的方法，专利申请号：200710063650.0
2. 一种分离棉花叶绿体DNA的方法，专利申请号：200710052665.7
3. 利用苦豆子分离基因SaVP1培育耐高温拟南芥的方法. 专利号 ZL 201310006089.8
4. 利用花花柴KcNHX1 基因培育耐高温拟南芥的方法专利申请号： 201310160491.1
5. 利用棉花GhPEPC基因创造棉花高油材料的方法专利申请号：201510836230.6
6. 一种提高棉花再生及转化效率的方法及应用专利申请号：201510836230.6
7. 一种棉花基因的编辑方法专利申请号 2016111884024
8. 一种DsRED2报告基因在棉花遗传改良中的应用专利申请号 201611113581.5

发表的论文及著作

[1]Luo, Jing; Liang, Sijia; Li, Jianying; Xu, Zhongping; Li, Lun; Zhu, Bangqin; Li, Zhe; Lei, Chaoliang; Lindsey, Keith; Chen, Lizhen*; Jin, Shuangxia*; Zhang, Xianlong. A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double- stranded RNA (dsRNA) homologous to Fatty acyl-CoA reductase (FAR) in cotton. New Phytologist,DOI:10.1111/nph.14636, 2017（*通讯作者） (SCI IF: 7.2)

[2]Wang, Pengcheng; Zhang, Jun; Sun, Lin; Ma, Yizan; Xu, Jiao; Liang, Sijia; Deng, Jinwu; Tan, Jiafu; Zhang, Qing; Tu, Lili; Daniell, Henry; Jin, Shuangxia*; Zhang, Xianlong*. High efficient multi-sites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system,Plant Biotechnology Journal, Accepted,2017,（*通讯作者） (SCI IF: 6.1)

[3] Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey & Xianlong Zhang,2017, Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication，Nature Genetics, 49(4):579-587

[4]Chengzhen Liang, Zhaohong Meng, Zhigang Meng, Waqas Malik, Rong Yan,Khin Myat Lwin, Fazhuang Lin1, Yuan Wang1, Guoqing Sun, Tao Zhou, Tao Zhu,Jianying Li, Shuangxia Jin, Sandui Guo & Rui Zhang. GhABF2, a bZIP transcription factor,confers drought and salinity tolerance in cotton (Gossypium hirsutum L.),Scientific Reports, 2016,6:35040.(SCI IF: 5.2)

[5]Zhongping Xu , Jingwen Li , Xiaoping Guo , Shuangxia Jin*, Xianlong Zhang*.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.Scientific Reports, 2016, DOI: 10.1038/srep33342.（*通讯作者）(SCI IF: 5.2)

[6] Jianying Li#, Lizhen Zhu#, Joe Hull, Sijia Liang, Henry Daniell, Shuangxia Jin*, Xianlong Zhang. Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly). Plant Biotechnology Journal, 2016, DOI: 10.1111/pbi.12554. (SCI, IF:6.1）(通讯作者）

[7]Qing Wang§, Yi Zhu§, Lin Sun, Lebin Li, Shuangxia Jin* and Xianlong Zhang.Transgenic Bt Cotton Driven by the Green Tissue-specific Promoter Shows Strong Toxicity to Lepidopteran pests and Lower Bt Toxin accumulation in seeds. SCIENCE CHINA Life Sciences, Online, 2016, DOI: 10.1007/s11427-015-4920-6 (SCI IF: 1.7)（*通讯作者）

[8]Ping Luo, Yuxiao Shen, Shuangxia Jin, Shasha Huang, Xu Cheng, Zhen Wang, Penghui Li, Jian Zhao, Manzhu Bao, Guogui Ning.Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling. Plant Science, 2016,245,35–49.(SCI IF: 3.4)

[9]Shuangxia Jin and Henry Daniell.The Engineered Chloroplast Genome Just Got Smarter. Trends in Plant Science, 2015，20(10):622-640 （SCI,IF:12.93)

[10]Geng Tian, Linlin Cheng, Xuewei Qi, Zonghe Ge, Changying Niu, Xianlong Zhang, Shuangxia Jin* Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms. Int. J. Biol. Sci. 2015; 11(11):1296-1305.(SCI, IF:4.5)（*通讯作者）

[11]Jin Shuanxia,Singh, Dolendro,Li Lebin,Zhang, Xianlong and Daniell, Henry. Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthases genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation. Plant Biotechnology Journal, 2015,13 (3), 435-446.(SCI, IF:6.1)

[12]Lebin Li§•Yi Zhu§•Shuangxia Jin* •Xianlong Zhang.Pyramiding. Bt genes for increasing resistance of cotton to two major lepidopteran Pests: Spodoptera litura and Heliothis armigera. Acta Physiologiae Plantarum，2014, 36 (10), 2717-2727（SCI, IF:1.5) （通讯作者）

[13]Shuangxia Jin and Henry Daniell.Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stress by reducing reactive oxygen species. Plant Biotechnology Journal, 2014 (12),1274-1285 . (SCI, IF:6.3)

[14]KwangChul Kwon*，Dheeraj Verma*，Shuangxia Jin*，Henry Daniell.Export of proteins from chloroplasts induced by reactive oxygen species during biotic and abiotic stress. PLOS ONE, 2013，8(6): e67106. (*并列第一). (SCI, IF: 3.7)

[15] Dheeraj Verma , Shuangxia Jin, Anderson Kanagaraj, Nameirakpam D. Singh1, Jaiyanth Daniel1, Pappachan E Kolattukudy1, Michael Miller2 and Henry Daniell1* Expression of Fungal Cutinase and Swollenin in Tobacco Chloroplasts Reveals Novel Enzyme Functions and/or Substrates. PLOS ONE, 2013;8(2):e57187 (SCI, IF: 3.7)

[16] Guanze Liu, Xuelin Li, Shuangxia Jin, Xuyan Liu, Longfu Zhu, Yichun Nie, Xianlong Zhang.Overexpression of Rice NAC Gene SNAC1 Improves Drought and Salt Tolerance by Enhancing Root Development and Reducing Transpiration Rate in Transgenic Cotton. 2014. PLoS One,e86895

[17] Wang Y, Jin S, Wang M, Zhu L, Zhang X (2013) Isolation and Characterization of a Conserved Domain in the Eremophyte H+-PPase Family. PLoS ONE 8(7): e70099. (SCI IF 3.7)

[18] Shuangxia Jin, Xianlong Zhang1, Henry Daniell. Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens. Plant Biotechnology Journal 2012,10(3):313-327 (Cover Story, IF 6.3 ).

[19] Jin SX, liu GZ, Zhu HG, Yang XY and Zhang XL.Transformation of Upland Cotton (Gossypium hirsutum L.) with gfp Gene as a Visual Marker. Journal of Integrative Agriculture,2012, 11(6):910-919, (SCI)

[20]Liu G, Jin S, Liu X, Tan J, Yang X, Zhang X (2012) Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications. Plant Cell, Tissue Organ Cult 110(2):261273(SCI，IF:3.6 )

[21] Shuangxia Jin, Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ß-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters. Plant Physiology ,2011,155,222-235.(SCI， IF:6.5)

[22]Seung-Bum Lee, Baichuan Li, Shuangxia Jin and Henry Daniell.Expression and characterization of　antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to prevent sexual transmission of diseases. Plant Biotechnology Journal.20119.100-115.(SCI, IF: 6.3)

[23]Verma D, Kanagaraj A, Jin SX, Singh N, Kolattukudy P, Daniell H. Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars. Plant Biotechnology Journal,2010,8:332-350　(SCI,IF:6.3).

[24] Jin SX, Zhang XL, Zhu HG, Zhou ZL, Nie YC. Detection of somaclonal variation of cotton (Gossypium hirsutum) through RAPD, SSR markers, cytogenetic and flow cytometer analysis. Plant Cell Reports, 2008，27:1303–1316.(SCI)

[25]Shuangxia Jin, Xianlong Zhang, et al, Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biologia Plantarum, 2006 50(4):519-524 .(SCI)

[26]Shuangxia Jin, Xianlong Zhang（2006） An efficient grafting system for transgenic plant recovery in cotton (Gossypium hirsutum L.). Plant Cell, Tissue and Organ Culture, 85(5): 181-185 .(SCI)

[27]Shuangxia Jin, Xianlong Zhang （2005） Factors affecting stable transformation and plant regeneration during transforming embryogenic callus of Upland cotton (Gossypium hirsutum L.) via Agrobacterium tumefaciens, Plant Cell, Tissue and Organ Culture, 81(2): 229-237 .(SCI)

[28]Shuangxia Jin, Xianlong Zhang （2005） Function Analysis of PromoterTrapping System after Inserting Cotton (Gossypium hirsutum L.) Genome. J GENET GENOMICS （Acta Genetica Sinca）, 32(12):1266-1274.

[29] Xianlong Zhang and Shuangxia Jin (2007) Transgenic Cotton: An Overview（invited review. Transgenic Plant Journal, 2007, 1(1), 150-162.

[30] Fang Ding, Shuangxia Jin, Ni Hong, Yun Zhong, Qing Cao, Ganjun Yi and Guoping Wang. 2008. Vitriﬁcation–cryopreservation, an efﬁcient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Plant Cell Reports,27(2),241-250 (SCI）

[31] Sun Y Q, Zhang X L, Jin S X. Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion. Theor Appl Genet, 2004, 109: 472-479 (SCI）

[32] Sun Y Q, Zhang X L, Jin S X Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum Anderss). Plant Cell Tissue Organ Cult, 2003, 75: 247-253 (SCI）

[33] Wu J H, Zhang X L, Jin S X．Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genetypes of Chinese cotton．In vitro Cell Dev Biol-Plant, 2004, 40: 371-375 (SCI）

[34] GUO X, JIN S, ZHANG X（2007）Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration. Biologia Plantarum, 51(2): 242-248 (SCI）

[35] Xiyan Yang, Xianlong Zhang, Shuangxia Jin, Lili Fu, Linggang Wang Production and characterization of asymmetric hybrids between upland cotton Coker201 (Gossypium hirsutum) and wild cotton (Gossypium klozschianum Anderss),Plant Cell Tissure Organ Cult, 2007, 89:225–235 (SCI）

[36] Yang X, Guo X, Zhang X, Nie Y and Jin S. Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis. BIOLOGIA PLANTARUM, 51 (3): 533-537, 2007 (SCI）

[37] Xianlong Zhang，Shuangxia Jin, Diqiu Liu, Zhongxu Lin, Fanchang Zeng, Longfu Zhu, Lili Tu, Xiaoping Guo. Cotton Biotechnology: Challenge the Future for Cotton Improvement, In: Biotechnology in Crop Improvement, GP Rao (ed.), Studium Press, LLC, Houstan, Texas, USA. Pp. 241-301

[38] Lili Tu, Xianlong Zhang, Diqiu Liu, Shuangxia Jin, Jinglin Cao, Longfu Zhu, Fenglin Deng, Jiafu Tan, Cunbin Zhang, 2007, Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis, Chinese Science Bulletin 52: 3110-3117 (SCI）

中文文章：

[1] 周小凤，金双侠，李保成，余渝，宁新柱，王旭文 .初步研究不同激素对海岛棉体细胞胚胎发生的影响.中国农学通报2009,25(11):78-83

[2]朱华国, 涂礼莉, 金双侠, 徐理, 谭家福, 邓锋林, 张献龙.棉花细胞初始脱分化的基因差异表达分析.科学通报， 2008，53 （20），2483-492 (SCI）

[3] 曹景林张献龙金双侠杨细燕朱华国付莉莉.棉花高效体细胞胚发生及同步控制培养体系研究.作物学报,2008, 34(2): 224−231
[4]谢德意，金双侠，郭小平，张献龙．长江和黄河流域棉区棉花品种体细胞胚胎发生和植株再生能力研究，作物学报，2007，33（3）：394－400

[5] 谢德意，金双侠，郭小平，张献龙．棉花ipt基因遗传转化效率及优化成苗技术研究，作物学报，2007，33（5）

[6] 金双侠, 韩杰,刘小云,刘冠泽,王一娴,唐文鑫,张献龙.SDS-蛋白酶法分离棉花cpDNA及psbA基因启动子、终止子克隆.分子植物育种,2007,5, 683-689.

[7] 周小凤，张碧瑶，刘冠泽，高巍，余渝，邓福军，李保成，孔宪辉，张献龙, 金双侠*.新疆棉花高体细胞胚胎发生能力基因型的筛选，分子植物育种, 2007,6, 1-8(通讯作者）.

[8] 张献龙，孙玉强，吴家和，金双侠，聂以春，郭小平，2004，棉花细胞工程及新种质创造，棉花学报，16（6）：368-373