涂礼莉

涂礼莉 女，1997年考入华中农业大学植物科学技术学院作物遗传育种专业学习。2001-2007年在华中农业大学作物遗传改良国家重点实验室进行硕博阶段学习和研究，方向为棉花分子生物学。毕业后留校任教并继续在作物遗传改良国家重点实验室进行棉花纤维发育机制方面的研究。
            
棉纤维是棉花的主要产出形式，是重要的天然纤维。能让棉纤维又长又细又强是所有棉花育种和分子生物学研究者的目标。棉纤维是种子表皮毛，最长的植物单细胞之一，纤维素沉积达90%以上。棉纤维是研究细胞分化，伸长及次生壁合成的良好材料。我们的研究旨在解析棉纤维的发育机制，克隆优质纤维基因，为棉纤维的改良打下基础。目前的研究方向主要有以下4个方面：
            
1. Ca2+信号调控纤维伸长和次生壁合成机理
Ca2+/CaM信号系统在细胞发育过程非常重要，同时Ca2+可以和果胶结合影响细胞壁刚性，从而影响细胞的伸长。以转基因棉花为材料，通过上调和下调GhCaM7的表达，发现GhCaM7可以上调ROS促进纤维的早期伸长；抑制膜联蛋白Annexin GhAnn2的表达的转基因棉花，纤维变短细胞壁变薄，纤维顶端Ca2+内流降低且钙离子信号发生了变化。目前已将钙信号感应器转入到棉花中，我们将详细的研究Ca2+的动态变化，及Ca2+/CaM信号网络的关键节点及调控机制，并期望精细调控Ca2+来调控纤维的发育及品质。
            
2.类黄酮等次生代谢途径与纤维发育
育成五彩缤纷且品质优良的彩色棉花一直是棉花遗传育种研究者的一个重要目标。类黄酮是植物重要的次生代谢物质，与色素形成相关。棕色棉纤维色素物质可能主要是氧化的原花青素。棉花人工驯化过程中，类黄酮代谢途径在白色棉花中下调，但白色棉花表达谱数据显示类黄酮代谢相关基因在纤维发育过程中仍然非常活跃。类黄酮含量与纤维品质可能负相关，我们的研究表明柚皮素（NAR）和二氢山柰酚（DHK）可能是抑制纤维发育的主要类黄酮。类黄酮代谢和木质素代谢有共同的代谢前体，木质素代谢相关基因在纤维发育过程中也很活跃，有效调控类黄酮代谢的同时协调木质素代谢的水平可能会促进纤维发育。我们将通过转基因棉花对类黄酮代谢的关键基因表达调控，对类黄酮和纤维发育直接的关系进行详细的解析。
            
 3.纤维发育的激素、转录因子及miRNA调控网络
 激素的角色是发号司令，而转录因子及miRNA传递及修饰信号。我们主要关注小肽等激素，发现磺钠肽PSK通过调控K+及ROS促进纤维发育。还发现合适浓度的JA促进纤维伸长。我们克隆了纤维不同发育时期特异、优势表达的转录因子，其中已有的结果表明下调转录因子TCP的表达抑制纤维的伸长，表达谱结果表明TCP通过影响JA信号途径的基因的表达来调控纤维发育。通过小片段RNA和降解组测序及转基因棉花验证miRNA揭示了miRNAs及其靶标在棉花纤维发育中的功能，在纤维细胞快速伸长期特异抑制miRNA157导致了成熟纤维细胞变短。我们将在已有的研究基础上继续深入，勾画出纤维发育的调控网络。
            
4.细胞次生壁合成与纤维发育
棉花纤维5个连续而又先后重叠发育时期决定了纤维的品质。其中次生壁合成不仅决定了纤维的细度和强度还决定了纤维的长度。已有的研究表明次生壁的适当推后延长了纤维的伸长时间，成熟纤维变长。棉纤维次生壁属性和次生壁合成调控目前还鲜有研究，我们实验室已经得到了一系列细胞壁蛋白及多糖合成相关基因的转基因棉花。已发现其中一个细胞壁蛋白Expansin可以促进纤维变长变细，这个Expansin是海岛棉特异表达且缺失一个结构域。
           

国家自然科学基金面上项目：海岛棉纤维伸长相关expansin基因的功能验证及启动子分析（2009.1-2011.12），30871560，主持
            

国家自然科学基金面上项目：棉花纤维发育不同时期特异/优势启动子的克隆与验证（2011.1-2013.12），31071465，主持
            

国家自然科学基金重点项目：钙信号及活性氧调控棉花纤维伸长及品质形成机理研究（2013.01—2017.12）31230056，参加 
            
国家自然科学基金面上项目：类黄酮影响棉花纤维发育机理及棕色纤维色素形成探究 （2015.1-2018.12），31471540，主持
            

国家自然科学基金面上项目：细胞壁蛋白GbEXPATR结构解析及棉纤维细胞壁属性与纤维品质相关性探究（2017.1-2020.12），31671739，主持
            

新教师基金: 海岛棉纤维发育早期特异启动子PGbPDF的克隆与分析（2009.1-2011.12）主持
            
转基因专项子课题：转基因新技术新方法研究（2011.7-2014.12），2013ZX08010001-006，主持
  

863：海岛棉优质基因标记开发及高产抗病分子标记及新品种培育（2012.1-2015.12），2012AA101108-3，参加
            

武汉市晨光计划：海岛棉优质纤维基因的功能验证及在陆地棉中的应用（2011.7-2012.12）主持

张献龙，林忠旭，朱龙付，涂礼莉，金双侠，郭小平，“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖
            
张献龙，聂以春，朱龙付，林忠旭，郭小平，卢怀玉，杨国正，余宏章，涂礼莉，“强优势多抗杂交棉新品种“华杂棉H318”的选育与应用”获2012年湖北省科技进步一等奖
       

张献龙，杨细燕，金双侠，孙玉强，吴家和，涂礼莉，朱龙付，聂以春，郭小平，林忠旭,“棉花体细胞离体遗传操作技术与控制理论”获2012年高等学校科学研究优秀成果奖（科学技术）自然科学二等奖
      

张献龙，聂以春，朱龙付，郭小平，卢怀玉，刘立清，林忠旭，涂礼莉，杨国正，金双侠，杨细燕，邹勇，余宏章，袁道军，“棉花种质创新及强优势杂交棉新品种选育与应用” 2013年国家科技进步二等奖
            
张献龙，朱龙付，涂礼莉等等，“从棉花组织中抽提RNA的方法”2006获国家发明专利。专利号：ZL200410060637.6
            
涂礼莉，李阳，张献龙，朱龙付，邓锋林，“两个棉花纤维伸长期优势表达的启动子及应用”专利授权号： ZL201010582387.8

张献龙，谭家福，涂礼莉，朱龙付，邓锋林，“利用棉花基因GbF3H改变花瓣颜色”，专利申请号：ZL201010582448.0
     

张献龙，邓锋林，涂礼莉，朱龙付，谭家福，“两个棉花纤维发育起始优势表达的强启动子及其应用”，专利授权号：ZL201010582335.0
  

张献龙，李阳，涂礼莉：袁道军：“棉花细胞壁伸展蛋白基因GbEXPATR 及应用”，专利授权号：ZL201410073300.2

张献龙，韩杰，谭家福，涂礼莉：“一种棉花Phytosulfokine前体基因GhPSKP及制备方法和应用”，专利授权号：ZL 201210260715.1
            

涂礼莉：胡海燕，谭家福，张献龙：“一种纤维伸长期优势表达的启动子及制备方法和应用”，专利授权号：ZL 201210288775.4

 2018年

Haiyan Hu, MaojunWang, Yuanhao Ding, Sitao Zhu, Guannan Zhao, Lili Tu*, Xianlong Zhang. Transcriptomic repertoires depict the initiation of lint and fuzz fibers in cotton (Gossypium hirsutum L.), Plant Biotechnol J, 2018, doi: 10.1111/pbi.12844

Maojun Wang, Pengcheng Wang, Fan Liang, Zhengxiu Ye, Jianying Li, Chao Shen, Liuling Pei, Feng Wang, Jiang Hu, Lili Tu, Keith Lindsey, Daohua He *, Xianlong Zhang *. A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation, New Phytol, 2018, 217(1):163-178

Maojun Wang, Pengcheng Wang, Min Lin, Zhengxiu Ye, Guoliang Li, Lili Tu, Chao Shen, Jianying Li, Qingyong Yang, Xianlong Zhang. Evolutionary dynamics of 3D genome architecture following polyploidization in cotton, Nat Plants, 2018, 4(2):90-97

2017年

Kai Guo, Lili Tu, Yonghui He, Jinwu Deng, Maojun Wang, Hui Huang, Zhonghua Li, Xianlong Zhang. Interaction between calcium and potassium modulates elongation rate in cotton fiber cells, J Exp Bot, 2017, 68(18):5161-5175

Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey, Xianlong Zhang. Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication. Nat Genet, 2017, 49(4): 579-587

Hu H, He X, Tu L, Zhu L, Zhu S, Ge Z, Zhang X*. GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like. Plant J, 2016, 88(6):921-935

胡海燕, 刘迪秋, 李允静, 李阳, 涂礼莉*, 一个棉花纤维伸长期优势表达启动子pGhFLA1 的克隆与鉴定, 作物学报, 2017, 43(6): 849-854

2016年

Kai Guo, Lili Tu*, Pengcheng Wang, Xueqiong Du, Shue Ye, Ming Luo and Xianlong Zhang, 2016, Ascorbate alleviates Fe deficiency-induced stress in cotton (Gossypium hirsutum) by modulating ABA levels. 2016. Frontiers in Plant Science, doi: 10.3389/fpls.2016.01997

Kai Guo, Xueqiong Du, Lili Tu*, Wenxin Tang, Pengcheng Wang, Maojun Wang, Zhen Liu, Xianlong Zhang, 2016, Fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (Gossypium hirsutum). J Exp Bot, 67(11):3289-3301

Maojun Wang, Pengcheng Wang, Lili Tu , Sitao Zhu, Lin Zhang, Zhonghua Li, Qinghua Zhang, Daojun Yuan and Xianlong Zhang *, 2016, Multi-omics maps of cotton fibre reveal epigenetic basis for staged single-cell differentiation. Nucleic Acids Res, 44(9):4067-4079

Haiyan Hu, Xin He, Lili Tu , Longfu Zhu, Sitao Zhu, Zhonghe Ge, Xianlong Zhang *. GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like. Plant J, 2016，88(6):921-935

Yang Li, Lili Tu*, Filomena A Pettolino, Shengmei Ji, Juan Hao, Daojun Yuan, Fenglin Deng, Jiafu Tan, Haiyan Hu, Qing Wang, Danny J. Llewellyn, Xianlong Zhang, 2016, GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. Plant Biotechnol J, 14(3): 951–963

2015年

Yang Li, Lili Tu*, Zhengxiu Ye, Maojun Wang, Wenhui Gao, Xianlong Zhang, 2015, A cotton fiber-preferential promoter, PGbEXPA2, is regulated by GA and ABA in Arabidopsis. Plant Cell Rep, 34(9):1539-49

Maojun Wang, Daojun Yuan, Lili Tu , Wenhui Gao, Yonghui He, Haiyan Hu, Pengcheng Wang, Nian Liu, Keith Lindsey, Xianlong Zhang *. Long noncoding RNAs and their proposed functions in fibre development of cotton (Gossypium spp.). New Phytol, 2015, 207(4):1181-1197

Daojun Yuan, Zhonghui Tang, Maojun Wang, Wenhui Gao, Lili Tu , Xin Jin, Lingling Chen, Yonghui He, Lin Zhang, Longfu Zhu, Yang Li, Qiqi Liang, Zhongxu Lin, Xiyan Yang, Nian Liu, Shuangxia Jin, Yang Lei, Yuanhao Ding, Guoliang Li, Xiaoan Ruan, Yijun Ruan*, Xianlong Zhang *, 2015, The genome sequence of Sea-Island cotton (Gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres. Sci Rep, 5: 17662, doi: 10.1038/srep17662

2014年

涂礼莉, 谭家福, 郭凯, 李中华, 张献龙*, 2014, 类黄酮代谢途径与棉花纤维发育, 中国科学(生命科学) Vol. 44 Issue (8): 758-765 （综述）

Nian Liu, Lili Tu, Wenxin Tang, Wenhui Gao, Keith Lindsey, Xianlong Zhang*, 2014, Small RNA and degradome profiling reveals a role for miRNAs and their targets in the developing fibers of Gossypium barbadense. The Plant J, 80(2):331-44, DOI: 10.1111/tpj.12636

Juan Hao, Sheng Chen, Lili Tu*, Haiyan Hu, Xianlong Zhang, 2014, GhH2A12, a replication-dependent histone H2A gene from Gossypium hirsutum, is negatively involved in development of cotton fiber cells. Plant Cell Reports 33: 1711-21

Wenxin Tang, Yonghui He, Lili Tu*, Maojun Wang, Yang Li, Yong-Ling Ruan, Xianlong Zhang. 2014, Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex. Plant Mol Biol, 85(6):613-25

Jie Han, Jiafu Tan, Lili Tu*, Xianlong Zhang, 2014, A peptide hormone gene, GhPSK promotes fiber elongation and contributes to longer and finer cotton fiber, Plant Biotechnol J, 12: 861-871

Wenxin Tang, Lili Tu*, Xiyan Yang, Jiafu Tan, Fenglin Deng, Juan Hao, Kai Guo, Keith Lindsey, Xianlong Zhang* , 2014， The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating ROS production, New Phytologist, 202(2): 509-20

2013年及以前

Jiafu Tan, Maojun Wang, Lili Tu*, Yichun Nie, Yongjun Lin and Xianlong Zhang, 2013, The Flavonoid Pathway Regulates the Petal Colors of Cotton Flower. PLoS ONE 8(8): e72364. doi:10.1371/journal.pone.0072364

Jiafu Tan, Lili Tu, Fenglin Deng, Haiyan Hu, Yichun Nie, and Xianlong Zhang*, 2013, A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin, Plant Physiology, 162: 86-95

Juan Hao, Lili Tu, Haiyan Hu, Jiafu Tan, Fenglin Deng, Wenxin Tang, Yichun Nie and Xianlong Zhang*, 2012, GbTCP, a cotton TCP transcription factor, confers fibre elongation and root hair development by a complex regulating system. J Exp Bot, 63: 6267-6281

Fenglin Deng, Lili Tu, Jiafu Tan, Yang Li, Yichun Nie, Xianlong Zhang, 2012, GbPDF1 (Protodermal factor 1) is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2. Plant Physiology, 158: 890–904

Jiafu Tan, Lili Tu*, Fenglin Deng, Rui Wu, Xianlong Zhang, 2012, Exogenous Jasmonic Acid Inhibited Cotton Fiber Elongation. The Journal of Plant Growth Regulation, 31: 599–605

Yuan Daojun, Lili Tu, and Zhang Xianlong*. Generation, Annotation and Analysis of First Large-Scale Expressed Sequence Tags from Developing Fiber of Gossypium barbadense L. PLoS ONE, 2011,6(7):e22758

M. Farooq Hussain Munis, Lili Tu, Fenglin Deng, Jiafu Tan, Li Xu, Shicheng Xu, Lu Long and Xianlong Zhang*, 2010, A thaumatin-like protein (PR-5) gene of cotton (Gossypium barbadence L.) involved in fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco,Biochemical and Biophysical Research Communications，393: 38-44

Yunjing Li, Diqiu Liu, Lili Tu, Xianlong Zhang*, Li Wang, Longfu Zhu, Jiafu Tan, Fenglin Deng，2010，Suppression of GhAGP4 gene expression repressed the initiation and elongation of cotton fiber，Plant Cell Reports, 29: 193-202

Diqiu Liu, Lili Tu, Yunjing Li, Li Wang, Longfu Zhu, Xianlong Zhang*, 2008, Genes encoding Fasciclin-Like Arabinogalactan proteins are specifically expressed during cotton fiber development, Plant Mol Biol Rep, 26: 98-113

Diqiu Liu, Lili Tu, Li Wang, Yunjing Li, Longfu Zhu, Xianlong Zhang*, Characterization and expression of plasma and tonoplast membrane aquaporins in elongating cotton fibers, Plant Cell Rep, 2008, 27: 1385–1394

Lili Tu, Xian-Long Zhang*, Shao-Guang Liang, Di-Qiu Liu, Long-Fu Zhu, Fan-Chang Zeng, Yi-Chun Nie, Xiao-Ping Guo, Feng-Lin Deng, Jia-Fu Tan, Li Xu, 2007, Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development, Plant Cell Reports, 26: 1309-1320

Lili Tu, Xianlong Zhang*, Diqiu Liu, Shuangxia Jin, Jinglin Cao, Longfu Zhu, Fenglin Deng, Jiafu Tan, Cunbin Zhang, 2007, Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis, Chinese Science Bulletin 52: 3110-3117

Diqiu Liu, Xianlong Zhang*, Lili Tu, Longfu Zhu, Xiaoping Guo, 2006, Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and later fiber development stages in cotton, Molecular Biology, 40(5): 741-749

Zhu Long-Fu, Tu Li-Li , Zhen Fan-Chang, Liu Di-Qiu, Zhang Xian-Long*, 2005, An Improved Simple Protocol for Isolation of High Quality RNA from Gossypium spp. Suitable for cDNA Library Construction, 作物学报, 31(12)：1657-1659

涂礼莉，张献龙*等，2003，海岛棉NBS类型抗病基因类似物的起源、多样性及进化，遗传学报，30：1071-1077