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Cotton cytochrome P450 CYP82D regulates systemic cell death by modulating the octadecanoid pathway

更新时间:2014-11-06 09:56:57点击次数:字号:T|T
Longqing Sun, Longfu Zhu, Li Xu, Daojun Yuan, Ling Min & Xianlong Zhang. 2014. NATURE COMMUNICATIONS
Abstract

Plant oxylipins are derived from unsaturated fatty acids and play roles in plant growth and development as well as defence. Although recent studies have revealed that fatty acid metabolism is involved in systemic acquired resistance, the precise function of oxylipins in plant defence remains unknown. Here we report a cotton P450 gene SILENCE-INDUCED STEM NECROSIS(SSN), RNAi suppression of which causes a lesion mimic phenotype. SSN is also involved injasmonate metabolism and the response to wounding. Fatty acid and oxylipin metabolite analysis showed that SSN overexpression causes hyperaccumulation of hydroxide and ketodiene fatty acids and reduced levels of 18:2 fatty acids, whereas silencing causes an imbalance in LOX (lipoxygenase) expression and excessive hydroperoxide fatty acid accumulation. We also show that an unknown oxylipin-derived factor is a putative mobile signal required for systemic cell death and hypothesize that SSN acts as a valve to regulate HR on pathogen infection.