Abstract
Transcription
factors (TFs) play an important role in the regulation of plant growth and
development. The study of the structure and function of TFs represents a
research frontier in plant molecular biology. The findings of these studies
will provide significant information regarding genetic improvement traits in
crops. Currently, a large number of TFs have been cloned, and their function
has been verified. However, relatively few studies that genetically map TFs in
cotton are available. To genetically map TFs in cotton in this study, specific
primers were designed for TF genes that were published in the Plant Transcription
Factor Database. A total of 977 TF primers were obtained, and 31 TF polymorphic
loci were mapped on 15 cotton chromosomes. These polymorphic loci were clearly
preferentially distributed on chromosomes 5, 11, 19 and 20; and TFs from the
same family mapped to homologous cotton chromosomes. In-silico mapping
verified that many mapped TFs were mapped on their corresponding chromosomes or
their homologous chromosomes’ corresponding chromosomes in the diploid genomes.
QTL mapping for fiber quality revealed that TF-Ghi005602-2 mapped on Chr19 was
associated with fiber length. Eighty-five TF genes were selected for RT-PCR
analysis, and 4 TFs were selected for qRT-PCR analysis, revealing unique
expression patterns across different stages of fiber development between the
mapping parents. Our data offer an overview of the chromosomal distribution of
TFs in cotton, and the comparative expression analysis between Gossypium
hirsutum and G. barbadense provides a
rough understanding of the regulation of TFs during cotton fiber development.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422734/