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Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds.

更新时间:2016-03-24 19:18:54点击次数:字号:T|T
Qing Wang, Yi Zhu, Lin Sun, Lebin Li, Shuangxia Jin* and Xianlong Zhang. 2016. SCIENCE CHINA Life Sciences

                 Abstract

A promoter of the PNZIP (Pharbitis nil leucine zipper) gene (1.459 kb) was cloned from Pharbitis nil and fused  to  the  GUS (β-glucuronidase)  and Bacillus thuringiensis endotoxin (Cry9C) genes. Several  transgenic  PNZIP::GUS  and  PNZIP::Cry9C  cotton  lines  were  developed by Agrobacterium-mediated transformation. Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants. In contrast  GUS  staining  in  the  reproductive  structures  such  as  petals,  anther,  and immature seeds of PNZIP::GUS  cotton was very faint. Two transgenicPNZIP::Cry9C lines and one transgenic cauliflower mosaic virus (CaMV) 35S::Cry9C  line  were  selected  for  enzyme-linked  immunosorbent  assay  (ELISA)  and  insect  bioassays. Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from 24.6 to 45.5 μg  g(-1) fresh weight.  In  green  tissues  such  as  the  leaves,  boll  rinds,  and  bracts  of the PNZIP::Cry9C line,  the  Cry9C  protein  accumulated  up  to  50.2, 39.7, and 48.3 μg g(-1) fresh weight respectively. In contrast, seeds of the PNZIP::Cry9C line (PZ1.3) accumulated only 0.26 μg g(-1) fresh weight of the Cry9C protein, which was 100 times lower than that recorded for the seeds of the CaMV 35S::Cry9C line. The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm. The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds. These features should allay public concerns about the safety of transgenic foods. We propose the future utility of PNZIP as an economical, environmentally friendly promoter in cotton biotechnology.

 

http://www.ncbi.nlm.nih.gov/pubmed/26728504

DOI: 10.1007/s11427-015-4920-6 (IF: 1.7)

PMID:
 
26728504